Overview
The HM-MXP vortex cell crushing instrument adopts the principle of rapid oscillation to fully mix the cell suspension with the microbeads. Through the mutual shearing and collision between the microbeads and the microbeads and the cells, the cell wall is effectively crushed and the contents are released, and the wall-breaking effect can reach more than 80%. The equipment is suitable for research work in ordinary laboratories, and has the characteristics of simple operation and high processing efficiency.
Features
1. Low equipment requirements and easy operation
2. Can handle multiple samples simultaneously
3. Easy to maintain the integrity of the genome
4. Good for downstream purification
5. It can effectively solve the difficulties caused by cell crushing of different cells due to their structure and quantity: ① Leather RANSBURG positive cell wall is mainly composed of peptidoglycans and acidic polysaccharides, all kinds of yeast and fungal cell walls are mainly composed of polysaccharides and proteins, and its dense network structure is not easy to crush; ② Some samples are difficult to sample and the number is limited; ③ Improper wall breaking methods may lead to genome fragmentation, affecting subsequent experiments
Principle
The HM-MXP cell crushing instrument makes the cell suspension and microbeads fully mixed by rapid oscillation, and the microbeads and the cells shear and collide with each other to promote cell wall crushing and release the contents, thereby achieving efficient cell wall breaking effect.
Applications
Yeast, bacteria, algae and other cell crushing
Standards
Permissible Ambient Temperature: 5~ 40 ℃, relative humidity: 80%
Steps
1. Place the sample within the appropriate capacity of the device
2. Set the required Speed range (100~ 2800rpm)
3. Set Timer Range (0-99h59min)
4. Start the equipment for continuous operation
5. Wait for the wall to break and take out the sample.
Notice
• Ensure that the equipment is used within the Permissible Ambient Temperature (5~ 40 ° C) and relative humidity (80%) range
• Confirm voltage matching (200-240V/100-120V optional) and Frequency (50-60Hz) before use
• Note that the applicable capacity of the device is 8 * 2ml, do not overload it
• Avoid violent vibration during operation to ensure stable placement of the equipment
• Regularly check the operating status of the equipment to ensure safe operation
