Overview
The HUXI HZL-240 Molecular Hybridization Instrument is a modern laboratory equipment specially designed for nucleic acid molecular hybridization technology. It combines a precise temperature control system and a vertical rotation system to achieve complementary pairing of bases of DNA/RNA single strands at a specific temperature and Rotation speed to form heteroduplexes. The instrument can automate the whole process of baking, pre-hybridization, and membrane washing. It is widely used in the fields of cloning gene screening, gel staining and decolorization, microbial fostering, and enzyme-linked reaction incubation. It is a reliable equipment for molecular hybridization research in modern laboratories.
Features
1. Using hot air thermostatic convection technology, the temperature is accurate and uniform, and the current actual temperature is displayed in real time.
2. Stepping motor drive swirl/spin, smooth operation, Rotation speed precision, durability, to solve the problem of synchronous belt peeling.
3. Equipped with a large screen Touchscreen, you can point the swirl/spin spindle with one click, which is convenient for loading and unloading the hybrid tube.
4. The liner and bracket are made of anti-corrosion 304 stainless steel, equipped with 3C certified tempered Glass door and sealing device, and equipped with electronic sensors.
5. The screen dynamically displays the "open/close" status, and has the function of automatically stopping the door, which effectively improves safety protection.
6. The time and speed of shaking the base at the bottom are adjustable, compatible with hybrid bags and enzyme labels, etc., to achieve multi-purpose in one machine.
7. With 50 sets of Data storage and 5-segment programming combination functions, it can automatically perform layer experiments with different times, Rotation speed and temperature to improve experimental efficiency and accuracy.
8. Support temperature Linear dispersion waveform display, record real-time temperature control status, and reserve ModBus Communication interface for future expansion of computer control or multiple Connectivity functions.
Principle
The working principle of the molecular hybridization instrument is based on the principle of base complementary pairing of nucleic acid molecules. The instrument operates under specific temperature, Rotation speed and time conditions set by the user through its special temperature control system (hot air thermostatic convection) and vertical rotation system (stepper motor drive). This combination provides a uniform and precise temperature environment and mixing effect for the reaction system, which prompts the two nucleic acid single-strands (DNA or RNA) with homologous sequences to undergo annealing denaturation treatment, and finally form stable heterogeneous double-stranded molecules.
Applications
Molecular hybridization of nucleic acid, screening of cloned genes, decolorization of gels, fostering of microorganisms, incubation of enzyme-linked reactions, all-Basis Weight or half-Basis Weight PCR detection (combined with enzyme immunoassay), genetic diagnosis of viral and bacterial diseases Clinical testing
Standards
3C certification
Steps
1. Prepare the sample and hybridization tube, and load the reaction system into the hybridization tube.
2. Set the experimental parameters through the 7-inch TFT true color Touchscreen, including target temperature, spindle swirl/spin speed, Working time, etc., or call the pre-stored programming combination.
3. Install the loaded hybrid tube on the swirl/spin rack of the instrument, which can be assisted in loading and unloading by means of the pointing function.
4. Close the tempered Glass door, and the instrument will automatically start running after the door is closed.
5. During operation, Linear dispersion and current status can be monitored in real time through the screen.
6. After the experiment is completed, the instrument can automatically stop and open the door to remove the hybrid tube.
7. Experimental data can be saved for subsequent review or retrieval.
Notice
• Do not open the Glass door at will during the operation of the instrument. After opening the door, the swirl/spin function will automatically stop to ensure safety.
• Please use the specified φ 35mm specification hybrid tube (standard 240mm length, optional 150mm or 300mm) and ensure stable installation.
When setting the temperature, do not exceed the calibration range of the instrument (room temperature + 5 ° C to 100 ° C).
• The instrument should be connected to a 220VAV, 50HZ power supply, and ensure that the Power load is within 800W.
• When cleaning, please use a suitable detergent to wipe the stainless steel liner and shell to avoid liquid entering the electrical part.
• If you need to work continuously for a long time, you can set the Working time to "0".
• The reserved ModBus Communication interface is an optional function, please confirm the configuration if you want to use it.
